Did you know that Lasergene versions 16.0 and later feature a site-directed mutagenesis workflow utilizing Protean 3D and SeqBuilder Pro? Site-directed mutagenesis is a molecular biology method that is used to make specific changes to the DNA sequence of a gene.
Nature commonly evolves proteins to provide sufficient fold stability to optimize its activity—not its energetic stability. Lasergene’s site-directed mutagenesis workflow is designed to help improve the developability of a protein by predicting which mutations may lead to increased protein stability and developability.
We have recently created a use case (see this poster we presented) that replicates the findings of Lim, et al. 1994 (Proc. Natl. Acad. Sci. USA, 91:423-7). Lim made three specific mutations to the repressor protein cl. This protein is part of the lambda P50 operon (PDB ID: 1LMB), which also includes the genes rexa and rexb.
To see the complete use case and our results, click here.
Want to try the workflow with your own data?
Request a Lasergene free trial to try this workflow for yourself.
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